Optimized oxidoreductases for medium and large scale industrial biotransformations
Project Secretariat
Dr Marta Pérez-Boada
E-mail: MPBoada@cib.csic.es
Consejo Superior de Investigaciones Científicas (CSIC)
Biological Research Centre (CIB)
Calle Ramiro de Maeztu 9, E-28040 Madrid, Spain
Phone: 34 918373112
Fax: 34 915360432
Mobile: 34 650080476
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about us
bokuUniversity of Natural Resources and Life Sciences, Vienna, Austria (BOKU)

Roland Ludwig
Contact information
R. Ludwig, Department of Food Science and Technology, BOKU, Muthgasse 18, A-1190 Wien, Austria;

Phone: 43 1 476546149
Fax: 43 1 476546199
E-mail: roland.ludwig@boku.ac.at

Description of the group
The Food Biotechnology Laboratory belongs to the Vienna Institute of Technology at BOKU. The group has three senior members, Dr. Diemtar Haltrich, Dr. Clemens Peterbauer and Dr. Roland Ludwig, and focuses on the screening, characterization, engineering and application of oxidoreductases in biocatalysis and biosensors. The laboratory (800 m2) hosts a pilot-plant for fermentation and downstream processing and fully equipped microbiology, molecular biology and analytical laboratories.
The contribution to INDOX will be managed by Dr. Roland Ludwig. His field of expertise are extracellular fungal oxidoreductases – their biochemical characterization, structure elucidation, modeling, engineering by rational and directed evolution methods and bioprocess/reaction engineering. He has published ~80 articles on oxidoreductases and filed 5 patents in the field. Other members of the group directly participating in the project are: Dr. Christoph Sygmund a postdoc who works on the characterization of CDH; Dr. Roman Kittl will be involved in LPMO research; Cindy Lorenz, staff technician, will add expertise in analytical techniques; as well as PhD students working on CDH (Daniel Kracher, Alfons Felice) and LPMO (Stefan Scheiblbrandner).

Main role in INDOX
BOKU will focus on the screening, cloning, small-scale production and characterization of cellobiose dehydrogenase and copper-dependent LPMO from fungi. The work to be performed includes studying their mechanism and improving their technical applicability by rational design. The engineered enzyme variants will then be produced at large scale and evaluated in small laboratory reactors to study the multi-enzyme cascade reactions. Finally, optimization of the system for up-scaling by reaction engineering will be addressed.
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Project Coordinator
Prof. Angel T. Martínez
Biological Research Centre (CIB, CSIC)
Calle Ramiro de Maeztu 9, E-28040 Madrid, Spain.
E-mail: ATMartinez@cib.csic.es
Phone: 34 918373112
Fax: 34 915360432

Angel T. Martínez is Research Professor of CSIC at the group of Biotechnology for Lignocellulosic Biomass of CIB. He is member of ACS, ASM and TAPPI, with around 250 publications mainly related to oxidoreductases and microbial biotechnology.

His experience is supported by participation in more than 55 national and international research projects, in most of them as head researcher. Regarding EC Framework Programmes, he has coordinated projects of Industrial Biotechnology from FP3 to FP7, such as PEROXICATS (KBBE-2010-4-265397), BIORENEW (NMP2-CT-2006-026456), PELAS (QLK3-1999-00590), PITCH (QLK5-1999-01357) and AIR2 (CT93-1219).
Official webpage of indox [ industrialoxidoreductases ]. Optimized oxidoreductases for medium and large scale industrial biotransformations. This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under Grant Agreement nº: FP7-KBBE-2013-7-613549. © indox 2013. Developed by garcíarincón