
University of Natural Resources and Life Sciences, Vienna, Austria (BOKU)

Roland Ludwig
Contact information
R. Ludwig, Department of Food Science and Technology, BOKU, Muthgasse 18, A-1190 Wien, Austria;
Phone: 43 1 476546149
Fax: 43 1 476546199
E-mail:
roland.ludwig@boku.ac.at
Description of the group
The
Food Biotechnology Laboratory belongs to the Vienna Institute of Technology at
BOKU. The group has three senior members, Dr. Diemtar Haltrich, Dr. Clemens Peterbauer and Dr.
Roland Ludwig, and focuses on the screening, characterization, engineering and application of oxidoreductases in biocatalysis and biosensors. The laboratory (800 m2) hosts a pilot-plant for fermentation and downstream processing and fully equipped microbiology, molecular biology and analytical laboratories.
The contribution to
INDOX will be managed by Dr.
Roland Ludwig. His field of expertise are extracellular fungal oxidoreductases – their biochemical characterization, structure elucidation, modeling, engineering by rational and directed evolution methods and bioprocess/reaction engineering. He has published ~
80 articles on oxidoreductases and filed
5 patents in the field. Other members of the group directly participating in the project are: Dr.
Christoph Sygmund a postdoc who works on the characterization of CDH; Dr.
Roman Kittl will be involved in LPMO research;
Cindy Lorenz, staff technician, will add expertise in analytical techniques; as well as PhD students working on CDH (
Daniel Kracher,
Alfons Felice) and LPMO (
Stefan Scheiblbrandner).

Fermenter
Main role in INDOX
BOKU will focus on the screening, cloning, small-scale production and characterization of cellobiose dehydrogenase and copper-dependent LPMO from fungi. The work to be performed includes studying their mechanism and improving their technical applicability by rational design. The engineered enzyme variants will then be produced at large scale and evaluated in small laboratory reactors to study the multi-enzyme cascade reactions. Finally, optimization of the system for up-scaling by reaction engineering will be addressed.