Optimized oxidoreductases for medium and large scale industrial biotransformations
Project Secretariat
Dr Marta Pérez-Boada
E-mail: MPBoada@cib.csic.es
Consejo Superior de Investigaciones Científicas (CSIC)
Biological Research Centre (CIB)
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[2013-11-25] PEROXICATS Project
A group of fungi marked the end of the coal age 300 million years ago
300 million years ago, the Earth suddenly interrupted massive production of coal. This fact determined the end of the Carboniferous, a period of the Paleozoic Era that had started 60 million years before, characterized by the successive formation of large carbon beds arising from accumulation and burial of ancient trees growing up in vast marshy forests.

An international scientific team with participation of the Spanish Research Council (CSIC) has found out that the end of this coal age coincided with the origin of a group of highly specialized fungi. The results, published in the last number of Science, indicate that these organisms developed a system for the efficient decay of the vast extent of plant biomass that had colonized terrestrial habitats.

“These primitive organisms, basidiomycete type fungi, had developed a mechanism based on enzymes capable of degrading a barrier extremely recalcitrant until that moment: lignin. This polymer, present in wood, provides strength and rigidity to the trees and makes vessels impervious enabling distribution of water and nutrients throughout the plant”, explains one of the authors, CSIC researcher Ángel T. Martínez.

Researchers have made this finding after comparative analysis of 31 fungal genomes. The study has allowed determining the mechanism that was employed by these fungi to degrade lignin. “This process is based in the production of a type of complex proteins named peroxidases, acting in synergy with other oxidative enzymes. We managed to establish the evolutive pathway and chronology of the different types of peroxidases responsible for lignin biodegradation. Moreover, the results have revealed the existence of peroxidases barely known up to date”, says Martínez, working at the Biological Research Centre of CSIC.

Novel biocatalysts

The enzymes found could be used in the future development of new industrial biocatalysts. The new enzymes will be expressed in model microorganisms and further purified, characterized and modified by protein engineering techniques.

“The same biological agents responsible for coal production decrease during the Carboniferous could nowadays allow us developing biotechnology tools aimed to the sustainable production of biofuels and other products from the renewable feedstock provided by plant biomass”, points out CSIC researcher.
These studies will be carried out in the frame of the European Project PEROXICATS (www.peroxicats.org), coordinated by CSIC with participation of researchers from the Biological Research Centre (CSIC), the Institute of Natural Resources and Agrobiology (CSIC) and the Institute of Catalysis and Petroleochemistry (CSIC), amongst others. The project counts also with collaboration from a German university and two private companies.

Dimitrios Floudas, Manfred Binder, Robert Riley, Kerrie Barry, Robert A. Blanchette, Bernard Henrissat, Angel T. Martínez, Robert Otillar, Joseph W. Spatafora, Jagjit S. Yadav, Andrea Aerts, Isabelle Benoit, Alex Boyd, Alexis Carlson1, Alex Copeland2, Pedro M. Coutinho, Ronald P. de Vries, Patricia Ferreira, Keisha Findley, Brian Foster, Jill Gaskell, Dylan Glotzer, Paweł Górecki, Joseph Heitman, Cedar Hesse, Chiaki Hori, Kiyohiko Igarashi, Joel A. Jurgens, Nathan Kallen, Phil Kersten, Annegret Kohler, Ursula Kües, T. K. Arun Kumar, Alan Kuo, Kurt LaButti, Luis F. Larrondo, Erika Lindquist, Albee Ling1, Vincent Lombard, Susan Lucas, Taina Lundell, Rachael Martin, David J. McLaughlin, Ingo Morgenstern, Emanuelle Morin, Claude Murat, Laszlo G. Nagy, Matt Nolan, Robin A. Ohm, Aleksandrina Patyshakuliyeva, Antonis Rokas, Francisco J. Ruiz-Dueñas, Grzegorz Sabat, Asaf Salamov, Masahiro Samejima, Jeremy Schmutz, Jason C. Slot, Franz St. John, Jan Stenlid, Hui Sun, Sheng Sun, Khajamohiddin Syed, Adrian Tsang, Ad Wiebenga, Darcy Young, Antonio Pisabarro, Daniel C. Eastwood, Francis Martin, Dan Cullen, Igor V. Grigoriev, y David S. Hibbett. The Paleozoic origin of enzymatic lignin decomposition reconstructed from 31 fungal genomes. Science. 336: 1715-1719

Official webpage of indox [ industrialoxidoreductases ]. Optimized oxidoreductases for medium and large scale industrial biotransformations. This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under Grant Agreement nº: FP7-KBBE-2013-7-613549. © indox 2013. Developed by garcíarincón