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Total records: 126
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[ 2014 ] Pita M, Maté D, González-Pérez D, Shleev S, Fernández VM, Alcalde M, De Lacey AL Bioelectrochemical Oxidation of Water J. Am. Chem. Soc., 136: 5892-5895

[ 2014 ] Piumi F, Levasseur A, Navarro D, Zhou S, Macellaro G, Mathieu Y, Ropartz D, Ludwig R, Faulds CB, Record E A novel glucose dehydrogenase from the white-rot fungus Pycnoporus cinnabarinus: production in Aspergillus niger and physicochemical characterization of the recombinant enzyme. Appl. Microbiol. Biotechnol., 98: 10105-10118

[ 2014 ] Rico A, Rencoret J, del Río JC, Martínez AT, Gutiérrez A Pretreatment with laccase and a phenolic mediator degrades lignin and enhances saccharification of Eucalyptus feedstock Biotechnol. Biofuels, 7: 6

[ 2013 ] Babot ED, del Río JC, Kalum L, Martínez AT, Gutiérrez A Oxyfunctionalization of aliphatic compounds by a recombinant peroxygenase from Coprinopsis cinerea Biotechnol. Bioeng., 110: 2323-2332

[ 2013 ] Bey M, Zhou S, Poidevin L, Henrissat B, Coutinho PM, Berrin JG, Sigoillot JC Cello-oligosaccharide oxidation reveals differences between two lytic polysaccharide monooxygenases (family GH61) from Podospora anserina Appl. Environ. Microbiol., 79: 488-496

[ 2013 ] Carabajal M, Kellner H, Levin L, Jehmlich N, Hofrichter M, Ullrich R The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase J. Biotech., 168: 15-23

Lignin peroxidase (LiP) from Trametopsis cervina has an exposed catalytic tyrosine (Tyr-181)instead of the conserved tryptophan of other lignin-degrading peroxidases. Pristine LiP showed alag period in veratryl alcohol (VA) oxidation. However, LiP after turnover with H2O2/VA (VA-LiP)lacked this lag, and H2O2-pretreated LiP (H2O2-LiP) was inactive. MS analyses revealed that VALiPincludes one VA molecule covalently bound to the side-chain of Tyr-181, whereas H2O2-LiPcontains hydroxylated Tyr-181. No adduct is formed by the Y171N variant. Molecular dockingshowed that VA binding is favored by sandwich π stacking with Tyr-181 and Phe-89. EPRspectroscopy after peroxide activation of the pretreated LiPs showed other protein radicals than thetyrosine radical found in pristine LiP, which were assigned to a tyrosine/VA adduct radical in VALiPand a dihydroxyphenyalanine radical in H2O2-LiP. Both radicals are able to oxidize large lowredox-potential substrates, but H2O2-LiP is unable to oxidize high redox-potential substrates.Transient-state kinetics showed that the tyrosine/VA adduct strongly promotes (>100-fold)substrate oxidation by compound II, the rate-limiting step in catalysis. The novel activationmechanism is involved in ligninolysis, as demonstrated using lignin model substrates. This is thefirst report on autocatalytic modification, resulting in functional alteration, among class-IIperoxidases.