Optimized oxidoreductases for medium and large scale industrial biotransformations
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126
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[ 2018 ]
Ewing TA, van Noord A, Paul CE, van Berkel WJ A Xylenol Orange-Based Screening Assay for the Substrate Specificity of Flavin-Dependent para-Phenol Oxidases
Molecules, 23: 164-182
[ 2018 ]
Gygli G, de Vries RP, van Berkel WJ On the origin of vanillyl alcohol oxidases
Fungal Gen. Biol., 116: 24-32
[ 2018 ]
Leonhardt S, Büttner E, Gebauer AM, Hofrichter M, Kellner H Draft Genome Sequence of the Sordariomycete Lecythophora (Coniochaeta) hoffmannii CBS 245.38
Genome Announc., 6
[ 2018 ]
Martínez AT, Camarero S, Ruiz-Dueñas FJ, Martínez MJ Biological Lignin Degradation
Lignin Valorization: Emerging Approaches. Ed. Gregg Beckham. RSC: 199-225
[ 2018 ]
Ullrich R, Poraj-Kobielska M, Scholze S, Halbout C, Sandvoss M, Pecyna MJ, Scheibner K, Hofrichter M Side chain removal from corticosteroids by unspecific peroxygenase
J. Inorg. Biochem., 183: 84-93
[ 2017 ]
Acebes S, Ruiz-Dueñas FJ, Toubes M, Saez-Jimenez V, Pérez-Boada M, Lucas F, Martínez AT, Guallar V Mapping the Long-Range Electron Transfer Route in Ligninolytic Peroxidases
J. Phys. Chem. B, 121: 3946-3954
year2016
Characterization of a new aryl-alcohol oxidase secreted by the phytopathogenic fungus Ustilago maydis
Couturier M, Mathieu Y, Li A, Navarro D, Drula E, Haon M, Grisel S, Ludwig R, Berrin JG
Appl. Microbiol. Biotechnol., 100: 697-706
The discovery of novel fungal lignocellulolytic enzymes is essential to improve the breakdown of plant biomass for the production of second-generation biofuels or biobased materials in green biorefineries. We previously reported that Ustilago maydis grown on maize secreted a diverse set of lignocellulose-acting enzymes including hemicellulases and putative oxidoreductases. One of the most abundant proteins of the secretome was a putative glucose-methanol-choline (GMC) oxidoreductase. The phylogenetic prediction of its function was hampered by the few characterized members within its clade. Therefore, we cloned the gene and produced the recombinant protein to high yield in Pichia pastoris. Functional screening using a library of substrates revealed that this enzyme was able to oxidize several aromatic alcohols. Of the tested aryl-alcohols, the highest oxidation rate was obtained with 4-anisyl alcohol. Oxygen, 1,4-benzoquinone, and 2,6-dichloroindophenol can serve as electron acceptors. This GMC oxidoreductase displays the characteristics of an aryl-alcohol oxidase (E.C.1.1.3.7), which is suggested to act on the lignin fraction in biomass.
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[ industrialoxidoreductases ]. Optimized oxidoreductases for medium and large scale industrial biotransformations. This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under Grant Agreement nº: FP7-KBBE-2013-7-613549. © indox 2013. Developed by
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