Optimized oxidoreductases for medium and large scale industrial biotransformations
CLOSE
Project Secretariat
Dr Marta Pérez-Boada
E-mail: MPBoada@cib.csic.es
Consejo Superior de Investigaciones Científicas (CSIC)
Biological Research Centre (CIB)
Calle Ramiro de Maeztu 9, E-28040 Madrid, Spain
Phone: 34 918373112
Fax: 34 915360432
Mobile: 34 650080476
CLOSE
Private area
User:


Password:

publications
Total records: 126
Pages:    1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21  

[ 2017 ] Alcalde M When directed evolution met ancestral enzyme resurrection Microbial Biotechnol., 10: 22-24
[ 2017 ] Ayuso-Fernández I, Martínez AT, Ruiz-Dueñas FJ Experimental recreation of the evolution of lignin-degrading enzymes from the Jurassic to date Biotechnol. Biofuels, 10: 67
[ 2017 ] Carro J, Martínez A, Medina M, Martínez AT, Ferreira P Protein dynamics promote hydride tunnelling in substrate oxidation by aryl-alcohol oxidase Phys. Chem. Chem. Phys., 19: 28666-28675
[ 2017 ] González-Pérez D, Alcalde M The making of versatile peroxidase by directed evolution Biocatalysis and Biotransformation, doi: 10.1080/10242422.2017.1363190
[ 2017 ] Gygli G, Lucas F, Guallar V, van Berkel WJ The ins and outs of vanillyl alcohol oxidase: Identification of ligand migration paths PLoS Comput. Biol., 13
[ 2017 ] Martínez AT, Ruiz-Dueñas FJ, Camarero S, Serrano A, Linde D, Lund H, Vind J, Tovborg M, Herold-Majumdar OM, Hofrichter M, Liers C, Ullrich R, Scheibner K, Sannia G, Piscitelli A, Sener ME, Kılıç S, van Berkel WJ, Guallar V, et al. Oxidoreductases on their way to industrial biotransformations Biotechnol. Adv., 35: 815-831
year2016
Advanced Synthesis of Conductive Polyaniline Using Laccase as Biocatalyst
de Salas F, Pardo I, Salavagione HJ, Aza P, Amourgi E, Vind J, Martínez AT, Camarero S
PlosOne, 11

Polyaniline is a conductive polymer with distinctive optical and electrical properties. Its enzymatic synthesis is an environmentally friendly alternative to the use of harsh oxidants and extremely acidic conditions. 7D5L, a high-redox potential laccase developed in our lab, is the biocatalyst of choice for the synthesis of green polyaniline (emeraldine salt) due to its superior ability to oxidize aniline and kinetic stability at the required polymerization conditions (pH 3 and presence of anionic surfactants) as compared with other fungal laccases. Doses as low as 7.6 nM of 7D5L catalyze the polymerization of 15 mM aniline (in 24 h, room temperature, 7% yield) in the presence of different anionic surfactants used as doping templates to provide linear and water-soluble polymers. Aniline polymerization was monitored by the increase of the polaron absorption band at 800 nm (typical for emeraldine salt). Best polymerization results were obtained with 5 mM sodium dodecylbenzenesulfonate (SDBS) as template. At fixed conditions (15 mM aniline and 5mM SDBS), polymerization rates obtained with 7D5L were 2.5-fold the rates obtained with commercial Trametes villosa laccase. Moreover, polyaniline yield was notably boosted to 75% by rising 7D5L amount to 0.15 μM, obtaining 1g of green polyaniline in 1L-reaction volume. The green polymer obtained with the selected system (7D5L/SDBS) holds excellent electrochemical and electro-conductive properties displayed in water-dispersible nanofibers, which is advantageous for the nanomaterial to be readily cast into uniform films for different applications.

Official webpage of indox [ industrialoxidoreductases ]. Optimized oxidoreductases for medium and large scale industrial biotransformations. This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under Grant Agreement nº: FP7-KBBE-2013-7-613549. © indox 2013. Developed by garcíarincón