Optimized oxidoreductases for medium and large scale industrial biotransformations
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Project Secretariat
Dr Marta Pérez-Boada
E-mail: MPBoada@cib.csic.es
Consejo Superior de Investigaciones Científicas (CSIC)
Biological Research Centre (CIB)
Calle Ramiro de Maeztu 9, E-28040 Madrid, Spain
Phone: 34 918373112
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publications
Total records: 126
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[ 2017 ] Rencoret J, Pereira A, del Río JC, Martínez AT, Gutiérrez A Delignification and Saccharification Enhancement of Sugarcane Byproducts by a Laccase-Based Pretreatment Sustainable Chem. Eng., 5: 7145-7154
[ 2017 ] Rodríguez-Escribano D, de Salas F, Pardo I, Camarero S High-Throughput Screening Assay for Laccase Engineering toward Lignosulfonate Valorization Int. J. Mol. Sci., 18: 1793-1803
[ 2016 ] Acebes S, Fernandez-Fueyo E, Monza E, Lucas F, Almendral D, Ruiz-Dueñas FJ, Lund H, Martínez AT, Guallar V Rational Enzyme Engineering Through Biophysical and Biochemical Modeling ACS-Catalysis, 6: 1624-1629
[ 2016 ] Couturier M, Mathieu Y, Li A, Navarro D, Drula E, Haon M, Grisel S, Ludwig R, Berrin JG Characterization of a new aryl-alcohol oxidase secreted by the phytopathogenic fungus Ustilago maydis Appl. Microbiol. Biotechnol., 100: 697-706
[ 2016 ] de Salas F, Pardo I, Salavagione HJ, Aza P, Amourgi E, Vind J, Martínez AT, Camarero S Advanced Synthesis of Conductive Polyaniline Using Laccase as Biocatalyst PlosOne, 11
[ 2016 ] del Río JC, Prinsen P, Cadena EM, Martínez AT, Gutiérrez A, Rencoret J Lignin–carbohydrate complexes from sisal (Agave sisalana) and abaca (Musa textilis): chemical composition and structural modifications during the isolation process Planta, 243: 1143-1158
year2016
Towards preparative peroxygenase-catalyzed oxyfunctionalization reactions in organic media
Fernandez-Fueyo E, Ni Y, Gomez Baraibar A, Alcalde M, van Langen LM, Hollmann F
J. Mol. Cat. B, doi: 10.1016/j.molcatb.2016.09.013

The peroxygenase from Agrocybe aegerita (AaeUPO) has been evaluated for stereoselective oxyfunctionalization chemistry under non-aqueous reaction conditions.

The stereoselective hydroxylation of ethylbenzene to (R)-1-phenylethanol was performed in neat substrate as reaction medium together with the immobilized biocatalyst and tertBuOOH as oxidant.

Stability and activity issues still have to be addressed. Nevertheless, gram-scale production of enantiopure (R)-1-phenylethanol was achieved with respectable 90,000 turnovers of the biocatalyst.

Official webpage of indox [ industrialoxidoreductases ]. Optimized oxidoreductases for medium and large scale industrial biotransformations. This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under Grant Agreement nº: FP7-KBBE-2013-7-613549. © indox 2013. Developed by garcíarincón