Optimized oxidoreductases for medium and large scale industrial biotransformations
Total records:
126
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[ 2019 ]
Linde D, Ayuso-Fernández I, Ruiz-Dueñas FJ, Martínez AT Different fungal peroxidases oxidize nitrophenols at a surface catalytic tryptophan
Arch. Biochem. Biophys., 668: 23-28
[ 2019 ]
Serrano A, Sancho F, Viña-Gonzalez J, Carro J, Alcalde M, Guallar V, Martínez AT Switching the substrate preference of fungal aryl-alcohol oxidase: towards stereoselective oxidation of secondary benzyl alcohols
Catal. Sci. Technol., doi: 10.1039/C8CY02447B
[ 2019 ]
Viña-Gonzalez J, Jimenez-Lalana D, Sancho F, Serrano A, Martínez AT, Guallar V, Alcalde M Structure‐Guided Evolution of Aryl Alcohol Oxidase from Pleurotus eryngii for the Selective Oxidation of Secondary Benzyl Alcohols
Adv. Synth. Catal., 361: 2514-2525
[ 2018 ]
Carro J, Fernandez-Fueyo E, Fernández-Alonso C, Cañada J, Ullrich R, Hofrichter M, Alcalde M, Ferreira P, Martínez AT Self-sustained enzymatic cascade for the production of 2,5-furandicarboxylic acid from 5-methoxymethylfurfural
Biotechnol. Biofuels, 11: 86-96
[ 2018 ]
Carro J, Ferreira P, Martínez AT, Gadda G Stepwise Hydrogen Atom and Proton Transfers in Dioxygen Reduction by Aryl-Alcohol Oxidase
Biochemistry, doi: 10.1021/acs.biochem.8b00106
[ 2018 ]
Ewing TA, Kühn J, Segarra S, Tortajada M, Zuhse R, van Berkel WJ Multigram Scale Enzymatic Synthesis of (R)‐1‐(4′‐Hydroxyphenyl)ethanol Using Vanillyl Alcohol Oxidase
Adv. Synth. Catal., 360: 2370-2376
year2017
Delignification and Saccharification Enhancement of Sugarcane Byproducts by a Laccase-Based Pretreatment
Rencoret J, Pereira A, del Río JC, Martínez AT, Gutiérrez A
Sustainable Chem. Eng., 5: 7145-7154
Sugarcane bagasse and straw, two major agro-industrial byproducts generated by the sugarcane industry, contain significant amounts of carbohydrates that can be hydrolyzed and then converted into ethanol or other valuable compounds. However, access to them is limited by the presence of lignin, a recalcitrant polymer that protects cell-wall polysaccharides from enzymatic hydrolysis. This work demonstrates the ability of an enzymatic pretreatment, based on the laccase from Pycnoporus cinnabarinus, and 1-hydroxybenzotriazole as mediator, to remove and/or modify lignin in sugarcane bagasse and straw residues, improving their subsequent saccharification. Up to 27% and 31% decreases of relative lignin content in ground sugarcane bagasse and straw, respectively, were achieved by the laccase-mediator pretreatment followed by alkaline peroxide extraction. Moreover, the lignin removal directly correlated with improvements in enzymatic saccharification, increasing glucose releases by around 39% and 46% for bagasse and straw, respectively, compared with those of the corresponding controls. Lignin depolymerization and degradation were made evident in the 2D-NMR spectra by a significant reduction in the number of aliphatic side chains involved in the main β-O-4′ and β-5′ interunit linkages, together with a remarkable removal of p-hydroxyphenyl (H), guaiacyl (G), and syringyl (S) lignin units as well as the associated p-coumarates and ferulates, with respect to polysaccharides.
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[ industrialoxidoreductases ]. Optimized oxidoreductases for medium and large scale industrial biotransformations. This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under Grant Agreement nº: FP7-KBBE-2013-7-613549. © indox 2013. Developed by
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